recombinant mouse adam17 Search Results


recombinant mouse adam17  (R&D Systems)
92
R&D Systems recombinant mouse adam17
Recombinant Mouse Adam17, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant mouse adam17/product/R&D Systems
Average 92 stars, based on 1 article reviews
recombinant mouse adam17 - by Bioz Stars, 2026-02
92/100 stars
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adam17  (R&D Systems)
93
R&D Systems adam17
(A) Resting lymph nodes from C57BL/6J mice ( n =3 animals/group) were collected for tissue sectioning and stained with monoclonal antibodies to detect metalloproteinases (magenta) as indicated. The bottom right image shows the magnified region within the red dash line along with a white dotted contour denoting B cell follicle. Top right image shows sections with stained protease and the location of the location of the FDC networks (anti-CD35 staining, green). Scale bars: 200 μm. (B-C) LN cells from immunized or naive C57BL/6J mice ( n= 3/group) were isolated, permeabilized, stained with anti-protease antibody, and analyzed by flow cytometry for expression of the indicated metalloproteinases. (B) Representative histograms of ADAM10, <t>ADAM17,</t> and MMP14 expression amongst subcapsular macrophages, LECs, T-cells, DCs, B cells, and FDCs compared to signal from isotype control antibody. (C) The ratio of the MFI of anti-protease to isotype control antibody for each cell type. Shown are mean±s.d. (one-way ANOVA with post-hoc Dunnett test for pair-wise comparison against B cells for multiple pair-wise comparisons, ****p ≤ 0.0001, ***p ≤ 0.001, *p ≤ 0.05). (D-E) C57BL/6J mice (n=3/group) were injected with 10 µg eOD-60mer 40 for 6 hours or together with saponin adjuvant for 24 hours prior to harvesting the LNs for sectioning and immunohistochemical staining to detect indicated proteases. (D) Regions within slices of LNs injected with saponin adjuvant and antigen for 24 hours showing false color overlays of protease (white), degraded antigen (green) and degraded antigen that colocalizes with protease (red). Degradation of eOD-60mer 40 was determined by FRET. Scale bars: 100 μm. (E) The fraction of degraded antigen from total detected eOD-60mer 40 that colocalized with indicated proteases in LNs injected with antigen only or with adjuvant. Data collected from at least 6 tissue sections from 6 lymph nodes. Graph shows mean±s.d. (F) C57BL/6J mice (n=3/group) were immunized with 10 µg eOD-60mer 40 and 5 µg saponin adjuvant and 2 hours later LNs were vibratome sectioned into 250 µm thick live tissue slices. These tissues were incubated for 6 hours with metalloproteinase inhibitor alone or together with broad spectrum protease inhibitor or left untreated (control). Each point represents one region from one tissue section. Data collected from at least 10 tissue sections from 6 lymph nodes. Graph shows mean±s.d. (one-way ANOVA with post-hoc Tukey test for multiple pair-wise comparisons, *p ≤ 0.028, ****p ≤ 0.0001).
Adam17, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adam17/product/R&D Systems
Average 93 stars, based on 1 article reviews
adam17 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

Image Search Results


(A) Resting lymph nodes from C57BL/6J mice ( n =3 animals/group) were collected for tissue sectioning and stained with monoclonal antibodies to detect metalloproteinases (magenta) as indicated. The bottom right image shows the magnified region within the red dash line along with a white dotted contour denoting B cell follicle. Top right image shows sections with stained protease and the location of the location of the FDC networks (anti-CD35 staining, green). Scale bars: 200 μm. (B-C) LN cells from immunized or naive C57BL/6J mice ( n= 3/group) were isolated, permeabilized, stained with anti-protease antibody, and analyzed by flow cytometry for expression of the indicated metalloproteinases. (B) Representative histograms of ADAM10, ADAM17, and MMP14 expression amongst subcapsular macrophages, LECs, T-cells, DCs, B cells, and FDCs compared to signal from isotype control antibody. (C) The ratio of the MFI of anti-protease to isotype control antibody for each cell type. Shown are mean±s.d. (one-way ANOVA with post-hoc Dunnett test for pair-wise comparison against B cells for multiple pair-wise comparisons, ****p ≤ 0.0001, ***p ≤ 0.001, *p ≤ 0.05). (D-E) C57BL/6J mice (n=3/group) were injected with 10 µg eOD-60mer 40 for 6 hours or together with saponin adjuvant for 24 hours prior to harvesting the LNs for sectioning and immunohistochemical staining to detect indicated proteases. (D) Regions within slices of LNs injected with saponin adjuvant and antigen for 24 hours showing false color overlays of protease (white), degraded antigen (green) and degraded antigen that colocalizes with protease (red). Degradation of eOD-60mer 40 was determined by FRET. Scale bars: 100 μm. (E) The fraction of degraded antigen from total detected eOD-60mer 40 that colocalized with indicated proteases in LNs injected with antigen only or with adjuvant. Data collected from at least 6 tissue sections from 6 lymph nodes. Graph shows mean±s.d. (F) C57BL/6J mice (n=3/group) were immunized with 10 µg eOD-60mer 40 and 5 µg saponin adjuvant and 2 hours later LNs were vibratome sectioned into 250 µm thick live tissue slices. These tissues were incubated for 6 hours with metalloproteinase inhibitor alone or together with broad spectrum protease inhibitor or left untreated (control). Each point represents one region from one tissue section. Data collected from at least 10 tissue sections from 6 lymph nodes. Graph shows mean±s.d. (one-way ANOVA with post-hoc Tukey test for multiple pair-wise comparisons, *p ≤ 0.028, ****p ≤ 0.0001).

Journal: bioRxiv

Article Title: Spatially regulated protease activity in lymph nodes renders B cell follicles a sanctuary for retention of intact antigens

doi: 10.1101/2021.11.15.468669

Figure Lengend Snippet: (A) Resting lymph nodes from C57BL/6J mice ( n =3 animals/group) were collected for tissue sectioning and stained with monoclonal antibodies to detect metalloproteinases (magenta) as indicated. The bottom right image shows the magnified region within the red dash line along with a white dotted contour denoting B cell follicle. Top right image shows sections with stained protease and the location of the location of the FDC networks (anti-CD35 staining, green). Scale bars: 200 μm. (B-C) LN cells from immunized or naive C57BL/6J mice ( n= 3/group) were isolated, permeabilized, stained with anti-protease antibody, and analyzed by flow cytometry for expression of the indicated metalloproteinases. (B) Representative histograms of ADAM10, ADAM17, and MMP14 expression amongst subcapsular macrophages, LECs, T-cells, DCs, B cells, and FDCs compared to signal from isotype control antibody. (C) The ratio of the MFI of anti-protease to isotype control antibody for each cell type. Shown are mean±s.d. (one-way ANOVA with post-hoc Dunnett test for pair-wise comparison against B cells for multiple pair-wise comparisons, ****p ≤ 0.0001, ***p ≤ 0.001, *p ≤ 0.05). (D-E) C57BL/6J mice (n=3/group) were injected with 10 µg eOD-60mer 40 for 6 hours or together with saponin adjuvant for 24 hours prior to harvesting the LNs for sectioning and immunohistochemical staining to detect indicated proteases. (D) Regions within slices of LNs injected with saponin adjuvant and antigen for 24 hours showing false color overlays of protease (white), degraded antigen (green) and degraded antigen that colocalizes with protease (red). Degradation of eOD-60mer 40 was determined by FRET. Scale bars: 100 μm. (E) The fraction of degraded antigen from total detected eOD-60mer 40 that colocalized with indicated proteases in LNs injected with antigen only or with adjuvant. Data collected from at least 6 tissue sections from 6 lymph nodes. Graph shows mean±s.d. (F) C57BL/6J mice (n=3/group) were immunized with 10 µg eOD-60mer 40 and 5 µg saponin adjuvant and 2 hours later LNs were vibratome sectioned into 250 µm thick live tissue slices. These tissues were incubated for 6 hours with metalloproteinase inhibitor alone or together with broad spectrum protease inhibitor or left untreated (control). Each point represents one region from one tissue section. Data collected from at least 10 tissue sections from 6 lymph nodes. Graph shows mean±s.d. (one-way ANOVA with post-hoc Tukey test for multiple pair-wise comparisons, *p ≤ 0.028, ****p ≤ 0.0001).

Article Snippet: This reaction was carried out by incubating 1 μM of the quenched AZP probe with 1 μg/mL of recombinant MMP14 (918-MP-010, R&D Systems), ADAM17 (2978-AD-10, R&D Systems), ADAM10 (946-AD-020, R&D Systems), Cathepsin D (1029-AS-010, R&D Systems), Cathepsin S (50769-M08H, Sinobiologicals), or Cathepsin L (1515-CY-010, R&D Systems).

Techniques: Staining, Bioprocessing, Isolation, Flow Cytometry, Expressing, Control, Comparison, Injection, Adjuvant, Immunohistochemical staining, Incubation, Protease Inhibitor